Jump to content

Chelex 100


Recommended Posts

hi all!

 

i want to use chelex 100 sodium form (Sigma 50-100 dry mesh) in order to remove calcium traces from my protein but i dont know how to use it.

 

any suggestion is always wellcome.

 

cheers,

s

Link to comment
Share on other sites

Check Dunn, M.F., et al., Biochemistry, vol. 19, 718 (1980). There are variation with differences in speed and recovery, but if you do not really intend to build a column or something similar the described approach should work fine. Just remember that the binding is highly pH dependent.

Edited by CharonY
Link to comment
Share on other sites

  • 1 month later...

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.