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Enzyme purification: fill in the blanks


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Fill in the blanks.

 

The first workable fraction in the enzyme purification is the __________. Each subsequent successful purification step should show an increase in ____________ and very little decrease in __________________. To keep track of all proteins during each step, we would typically run a general protein assay, such as the ________, which utilizes the dye Coomassie Blue, which binds to ___________ groups in proteins.

 

What I have so far:

 

1. Crude

2. Not sure if it's specific activity or fold purification.

3.

4. Bradford assay

5. Hydrophobic

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