D_C_ Posted December 10, 2006 Share Posted December 10, 2006 Fill in the blanks. The first workable fraction in the enzyme purification is the __________. Each subsequent successful purification step should show an increase in ____________ and very little decrease in __________________. To keep track of all proteins during each step, we would typically run a general protein assay, such as the ________, which utilizes the dye Coomassie Blue, which binds to ___________ groups in proteins. What I have so far: 1. Crude 2. Not sure if it's specific activity or fold purification. 3. 4. Bradford assay 5. Hydrophobic Link to comment Share on other sites More sharing options...
shel Posted December 20, 2006 Share Posted December 20, 2006 2 should definitely be specific activity. I would guess that 3 is total solute concentration but I'm not so sure Link to comment Share on other sites More sharing options...
Bluenoise Posted December 20, 2006 Share Posted December 20, 2006 I'd say 3 is target concentration for lack of a better word. Link to comment Share on other sites More sharing options...
ho&ho Posted December 21, 2006 Share Posted December 21, 2006 2.specific activity or purifiction (i think both r right) 3. yield Link to comment Share on other sites More sharing options...
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