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SDS-Page Advantages


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You are comparing two entirely different tools. A PAGE does separation, whereas a MS is basically a m/z analyzer (if we ignore specialty things like ion mobility). It does not really do any separation by itself. In fact, a common workflow is to couple SDS PAGE with subsequent MS for protein identification.

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  • 2 weeks later...

The answer is price. SDS-PAGE is waaaaay cheaper. Fun fact - LC-MS systems cost a fortune; one can buy himself a good house on such money. From this also follows an issue of sample preparation - one doesn't care if sample is 'dirty' while conducting SDS-PAGE, mistakes are cheap. It is not so in the case of LC-MS, where care must be taken to provide only 'right' samples.

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