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Enzyme calculation


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#1 John.matheson

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Posted 11 January 2017 - 01:45 PM

Hi All,

 

Looking for some advice.

 

If I have a protease liquid enzyme of unknown activity how do I calculate activity in U/ml using a colorimteric substrate?

 

All best,

 

Niall


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#2 ecoli

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Posted 11 January 2017 - 07:36 PM

Assuming you have an spectrophotometer, you make a linear standard with a defined product and measure optical density at the optimum wavelength.


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#3 BabcockHall

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Posted 11 January 2017 - 11:19 PM

Is this homework?  Do you know what the definition of activity is?


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