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how to determine amount of proteins in horse's blood ?


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Amount as in different types of proteins? Electrophoresis does not have the resolving power to do that (and even 2D would be too limited). Or are you talking about total abundance? In the latter case any protein quantification method (Lowry, BCA, Coomassie etc.) would work. Most of it is going to be albumin, anyway with a decent chung also being hemoglobin, IgGs and so on.

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