shanglei1986 Posted January 20, 2017 Share Posted January 20, 2017 For example, a kind of enzyme is characterized by the N-terminal Serine/Threonine kinase. And my question is how to find the kinase activity site, how to make the kinase dead with one site mutation without any reference. Link to comment Share on other sites More sharing options...
CharonY Posted January 20, 2017 Share Posted January 20, 2017 There are several databases that can assist you in that, though of course without actual experimental verification they all will just be predictions based on sequence similarities. Uniprot is a general database that aggregates a lot of this information. If you want to implement something yourself you can look into protein family/domain databases such as pfam, prosite, etc. But again, usually you would knock out the whole domain, or at least a sizeable chunk of it. Figuring out the required minimum mutation to deactivate a given activity is quite a bit trickier. Link to comment Share on other sites More sharing options...
shanglei1986 Posted January 24, 2017 Author Share Posted January 24, 2017 There are several databases that can assist you in that, though of course without actual experimental verification they all will just be predictions based on sequence similarities. Uniprot is a general database that aggregates a lot of this information. If you want to implement something yourself you can look into protein family/domain databases such as pfam, prosite, etc. But again, usually you would knock out the whole domain, or at least a sizeable chunk of it. Figuring out the required minimum mutation to deactivate a given activity is quite a bit trickier. Thanks for your reply Link to comment Share on other sites More sharing options...
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